Andrea KoenigAndrea L. Koenig, Ph.D.
Colorado School of Mines
Division of Environmental Science and Engineering
1500 Illinois Street
Golden, CO 80401
Phone: (303) 506 4774
Fax: (303) 273 3413
akoenig@mines.edu

 

Education:

1998: Ph.D., Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA

1994: M.S., Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA

1992: B.A., Biochemistry, Occidental College, Los Angeles, CA

Professional Experience:

03/08-present: Research Scientist, Colorado School of Mines, Golden, CO

09/07-03/08: Research and Business Consultant, Gc-Free, Inc., San Diego, CA

05/04-06/07: Research Scientist, Department of Medicine, Denver Health, Denver, CO

2002-2004:  Research Scientist/Post-doctoral fellow, Biochemistry/Surface Science, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland

1999-2002: Post-doctoral fellow, Chemistry, Colorado State University, Ft. Collins, CO

1998-1999: Post-doctoral fellow, Microbiology, Colorado State University, Ft. Collins, CO

1992-1998:  Research Assistant, UC-San Diego, La Jolla, CA

1992-1994:  Teaching Assistant, UC-San Diego, La Jolla, CA

1990-1992:  Teaching Assistant, Occidental College, Los Angeles, CA

1989-1992:  Research Assistant, Occidental College, Los Angeles, CA

Peer-Reviewed Publications:

Andrea L. Koenig, Doug Looker and Gary Rosenthal. Variability among different lots of unfractionated and low molecular weight heparins in blocking L- and P-selectin binding to SLex. In submission. February, 2009.

Didier Falconnet, Andrea L. Koenig, Fabiano Assi and Marcus Textor. A combined photolithographic and molecular assembly approach to produce functional micropatterns for applications in the biosciences. Advanced Functional Materials, 14: 749-756 (2004).

Andrea L. Koenig, Veronica Gambillara, and David W. Grainger. Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates. J. Biomed. Mater. Res. 64A: 20-37 (2003).

Andrea M. Rediske, Andrea L. Koenig, Nazir Barekzi, Linda Cash Ameen, David W. Grainger, and Jeffrey B. Slunt. Polyclonal human antibodies reduce bacterial attachment to soft contact lens and corneal cell surfaces. Biomaterials, 23: 4565-4572 (2002).

Andrea L. Koenig and David W. Grainger. “Cell Adhesion-Dependent Signaling Pathways on Biomaterials Surfaces, “ in Biomimetic Materials and Design: Interactive Biointerfacial Strategies, Tissue Engineering and Targeted Drug Delivery. A. Dillow and A. Lowman, Editors. Marcel-Dekker, Inc., New York. 2002, pp. 187-250.

Andrea L. Koenig and David W. Grainger. “Cell-Synthetic Surface Interactions: Targeted Cell Adhesion,” in Methods of Tissue Engineering. A. Atala and R. Lanza, Editors. Academic Press, San Diego. 2001, pp. 751-770.

Rakesh K. Jain, Conrad F. Piskorz, Bao-Guo Huang, Robert D. Locke, Hui-Ling Han, Andrea Koenig, Ajit Varki, and Khushi Matta. Inhibition of L- and P-Selectin by a Rationally Synthesized Novel Core 2-Like Structure Containing GalNAc-Lewisx and Neu5Ac2-3Gal1- 3GalNAc Sequences. Glycobiology. 8: 707-717 (1998).

Andrea Koenig, Karin Norgard-Sumnicht, Robert Linhardt, and Ajit Varki. Differential Interactions of Heparin and Heparan Sulfate Glycosaminoglycans with the Selectins. Implications for the use of Unfractionated and Low Molecular Weight Heparins as Therapeutic Agents. J. Clin. Invest., 101: 877-899 (1998).

Andrea Koenig, Rakesh Jain, Rakesh Vig, Karin E. Norgard-Sumnicht, Khushi L. Matta, and Ajit Varki. Selectin Inhibition: Synthesis and evaluation of novel sialylated, sulfate, and fucosylated oligosaccharides, including the major capping group of GlyCAM-1. Glycobiology. 7: 79-93 (1997).

Brian J. Hicke, Susan R. Watson, Andrea Koenig, C. Kate Lynott, Robert F. Bargatze, Ying-Fon Chang, Steven Ringquist, Lotus Moon-McDermott, Susan Jennings, Tim Fitzwater, Hui-Ling Han, Nissi Varki, Irma Albinana, Michael C. Willis, Ajit Varki, and David Parma. DNA Aptamers Block L-Selectin Function In Vivo. J. Clin. Invest., 98: 2688-2692 (1996).

Dan O’Connell, Andrea Koenig, Susan Jennings, Brian Hicke, Hui-Ling Han, Tim Fitzwater, Ying-Fon Chang, Nissi Varki, David Parma, and Ajit Varki. Calcium-dependent oligonucleotide antagonists specific for L-selectin. Proc. Natl. Acad. Sci., USA, 93: 5883-5887 (1996).

Koenig, Andrea L., Alicia J. Hagar, and Tetsuo Otsuki. Synthesis of 4’ Halomethyl-4,5’,8 Trimethylfurocoumarin. It’s Unusual Substitution Reaction. Chemistry Express, 8: 463-466 (1993).

Conference Presentations:          

Poster Presentation at the Swiss Competence in Materials Conference: Burgdorf, Switzerland, CH-3400, 04/03

Poster Presentation at Gordon Research Conference on Biomaterials: Biocompatibility and Tissue Engineering, Holderness, NH, 07/01

Poster Presentation at 27th Annual Society for Biomaterials Meeting and Exposition, Saint Paul, MN, 04/01

Poster Presentation at the American Society of Hematology, San Diego, CA, 12/97

Poster Presentation at the Glycobiology 1997 Meeting, Long Beach, CA, 11/97

Poster Presentation at Biomedicine ‘96 Medicinal Research Meeting, Washington D.C., 05/96

Speaker at the 201st ACS National Meeting and Exposition Program, Atlanta, GA, 04/91

Poster Presentation at the 23rd Reaction Mechanisms Conference, University of Colorado-Boulder, Boulder, CO, 04/90

Membership in Professional Societies:

Associate member in the Society of Regenerative Medicine and Stem Cell Biology, Inc., 2001-2004.

Patents:

U.S. Patent #6596705:  Inhibition of L-Selectin and P-Selectin Mediated Binding Using Heparin.  Authors:  Varki, A. and Koenig, A.  International Class:  A61K 031/727.  Product(s) licensed and in development through the University of California-San Diego Technology Transfer Office to two companies.

U.S. Patent #6787365:  Inhibition of L-Selectin and P-Selectin Mediated Binding Using Heparin.  Authors:  Varki, A. and Koenig, A.  International Class:  G01N 33/00 (A61K 31/70). Product(s) licensed and in development through the University of California-San Diego Technology Transfer Office to two companies.

U.S. Patent Filed #WO9801140A1:  Oligonucleotides as Inhibitors of Selectins.  Authors:  Varki, A. and Koenig, A.

Teaching Experience:

At ETH-Zürich:  Responsible for supervising the projects of three Ph.D. students (Dr. Marc Dussellier, Dr. Didier Falconnet and Daniela Pasqui) and two M.S. students (Miribai Koch and Christian Brunner) (2002-2004).

At CSU: Responsible for the training of an engineering M.S. student (Veronica Gambillara) from ETH-Zürich (2000), an electrical engineering B.S. student (J. Brach) from CSU (2002), a mechanical engineering M.S. student (Brandon Santoni) from CSU (2002) and a clinical veterinary science D.V.M./M.S. student (Dr. Tatiana Motta) from CSU (2002).

At UCSD: Teaching assistant for General Chemistry (Fall, 1993) and for Organic Chemistry Laboratory (1994-1996).

At Occidental: Teaching assistant for Molecular Biology Laboratory (Winter, 1998).

At La Canada High School, La Canada, CA: Teaching assistant for advanced students in Sixth Grade Science (Summer, 1998).

Research Support.

Ford Foundation Summer Scholarship, Conducted organic synthesis of psoralens, DNA-intercalating molecules, for the treatment of psoriasis. 1990.
Role:  Award recipient

Academic-Industrial Undergraduate Research Partnership (AIURP) Fellowship, sponsored by Eli Lilly and Company, Continued work on SN1 and SN2 mechanisms of the synthesis of furocoumarin, a DNA-intercalating molecule, for the treatment of psoriasis. 1991.
Role:  Award recipient

Trainee Investigator Award for Excellence in Scientific Research, sponsored by AAP, ASCI, and
AFCR, Study of three families of carbohydrates as novel ligands for the selectins, which have been shown to block tumor metastasis and suppress autoimmune diseases. 1996.
Role:  Award recipient

NIH Training grant, No. T32 DK07202, Continued work on small blood-group carbohydrates and glycosaminoglycans (GAGs) as selectin inhibitors and potential therapeutics for treatment of autoimmune diseases. 5/97 – 5/98
Role:  Award recipient

WHO grant Chatterjee, D. (Principal Investigator).  Isolation of lipoarabinomannan (LAM) and arabinogalactin (AG) from two strains (prior to and after drug treatment) of M. Avium complex (MAC) originally from an AIDS patient with disseminated MAC infection. 12/98 – 06/99.
Role:  Co-Investigator

RO1 NIH/NIAID Grainger, D. (Principal Investigator), No. R01 GM56751-01. Surface chemistry and protein biochemistry on endothelial cell signaling upon cell attachment to polymer substrates for the study of implant biomaterials and their effects on human tissues.  07/99 – 08/02.
Role:  Co-Investigator

Colorado RNA Center Small Grant Award, Use of RT-PCR to look at the presence of fibronectin (FN) in cells that bind to fluorocarbon (similar to Teflon), hydrophobic and hydrophilic implant biomaterials. 02/01.
Role:  Principal Investigator

Colorado Commission on Higher Education (CCHE) Seed Grant, Protein analysis and cell culture on calcium phosphate microcarriers and their controlled release of OP-1 for bone regeneration. 01/02
Role: Principal Investigator

NIH Toribara, N. (Principal Investigator) No. P01A146518  Expression of siRNAs in neuroblastoma cells that cause cell resistance to differentiation or apoptosis in order to treat neuroblastomas.  Genes targeted by these populations of siRNAs will be identified and characterized. 05/04 – 06/07.
Role:  Co-Investigator

Adult General Clinical Research Center (GCRC) Grant, Analysis of the DNA promoter sequences that control the expression of gastric secreted mucins, MUC2, MUC5AC, MUC5B and MUC6, to examine their protective role in the stomach. 11/04.
Role: Principal Investigator

Research and consultant contract with Noble Molecules (Boulder, CO),  Screening various lots of commercially available low molecular weight and unfractionated heparins for their ability to block selectin binding to immobilized SLex.  Heparins with low IC50 values in ELISAs show potential as therapeutics for autoimmune diseases and cancer metastasis.  08/06.
Role: Principal Investigator

Research contract with Ciris Energy, Inc. (Denver, CO).  Marr, J. and Figueroa, L. (Principal Investigators) Isolation of anaerobic archaea, in particular methanogens, in order to identify which species are present using molecular biology techniques (qPCR and cloning/sequencing the conserved 16S ribosomal gene sequence). The goal is to discover which methanogens are present in these environmental samples and how we can supplement their growth for methane production for use as an eco-friendly energy source.
Role:  Co-Investigator

Other Interests: